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Scanpy
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| # | Skill | Repository | Stars | Used in | Tokens | Auto-check | Licence | Updated |
|---|---|---|---|---|---|---|---|---|
| 1 | Performs quality control on single-cell RNA-seq data (.h5ad or .h5 files) using scverse best practices with MAD-based filtering and comprehensive visualizations. | FreedomIntelligence/ | 3.1k | 2 repos | ~2k | Automated safety check: Pass | Apache-2.0 | 2 mo ago |
| 2 | Walks through single-cell RNA-seq analysis with Scanpy: loading .h5ad and 10X data, QC, normalization, PCA and UMAP, Leiden clustering, marker genes and cell type annotation. | davila7/ | 32k | 15 repos | ~2.8k | Automated safety check: Pass | MIT | today |
| 3 | Reproducible Scanpy workflow for human or mouse 10x scRNA-seq and snRNA-seq count matrices: single-sample descriptive QC, clustering and annotation, or comparative donor-aware pseudobulk DE and Milo… | PKU-YuanGroup/ | 620 | — | ~1.3k | Automated safety check: Pass | MIT | today |
| 4 | 4.Anndata This skill should be used when working with annotated data matrices in Python, particularly for single-cell genomics analysis, managing experimental measurements with metadata, or handling… | davila7/ | 32k | 11 repos | ~2.5k | Automated safety check: Pass | MIT | today |
| 5 | Query CZ CELLxGENE Census (61M+ cells). An agent skill from davila7/claude-code-templates. | davila7/ | 32k | 11 repos | ~3.8k | Automated safety check: Pass | MIT | today |
| 6 | Prepares bulk RNA-seq FASTQ, Salmon, STAR or featureCounts output for gene-level differential expression. | K-Dense-AI/ | 48k | 1 repo | ~4.2k | Automated safety check: Pass | MIT | 3 days ago |
| 7 | Performs pathway and gene-set enrichment analysis on gene lists or ranked gene data and interprets the results. | K-Dense-AI/ | 48k | 1 repo | ~4.2k | Automated safety check: Pass | MIT | 3 days ago |
| 8 | 8.Scvelo Performs RNA velocity analysis with scVelo from spliced and unspliced single-cell RNA counts. | K-Dense-AI/ | 48k | 1 repo | ~3.1k | Automated safety check: Pass | BSD-3-Clause | 3 days ago |
| 9 | 9.Scanpy Performs Scanpy single-cell RNA-seq QC, normalization, HVG selection, PCA/UMAP/t-SNE, clustering, exploratory marker ranking, pseudobulk preparation, visualization, and Seurat or… | K-Dense-AI/ | 48k | 1 repo | ~5.1k | Automated safety check: Pass | BSD-3-Clause | 3 days ago |
| 10 | Queries the CZ CELLxGENE Census programmatically for versioned public single-cell and spatial transcriptomics data. | K-Dense-AI/ | 48k | 1 repo | ~3.4k | Automated safety check: Notes | MIT | 3 days ago |
| 11 | 11.Scvi Tools Fits probabilistic models for single-cell omics, including scVI batch integration, scANVI annotation, totalVI CITE-seq, MultiVI RNA/ATAC integration, and posterior differential expression. | K-Dense-AI/ | 48k | 1 repo | ~2.6k | Automated safety check: Pass | BSD-3-Clause | 3 days ago |
| 12 | 12.Omics Tools Omics and single-cell workflow guide for AnnData, Scanpy-style dataset profiling, PyDESeq2-oriented count checks, pysam alignment inspection, and pyOpenMS mass-spectrometry summaries. | DrugClaw/ | 125 | — | ~1.1k | Automated safety check: Pass | Apache-2.0 | 6 mo ago |
| 13 | 13.Scvi Tools Probabilistic single-cell RNA-seq with scvi-tools — scVI for a batch-corrected latent space, scANVI for semi-supervised label transfer, and Bayesian differential expression. | JimLiu/ | 227 | 4 repos | ~2.1k | Automated safety check: Pass | Apache-2.0 | 3 mo ago |
| 14 | 14.Anndata Handles annotated matrices in single-cell analysis, .h5ad and Zarr files, and integration with the scverse ecosystem. | K-Dense-AI/ | 48k | 1 repo | ~3.9k | Automated safety check: Notes | BSD-3-Clause | 3 days ago |
| 15 | Annotated matrices for single-cell genomics. An agent skill from jaechang-hits/SciAgent-Skills. | jaechang-hits/ | 371 | 2 repos | ~5.8k | Automated safety check: Pass | BSD-3-Clause | 10 days ago |
| 16 | Local Scanpy pipeline for single-cell RNA-seq QC, optional doublet detection, clustering, marker discovery, optional CellTypist annotation, optional latent downstream mode from… | ClawBio/ | 1.2k | 1 repo | ~2.9k | Automated safety check: Pass | MIT | today |
| 17 | Produce and interpret PCA, t-SNE, UMAP, and PHATE plots for high-dimensional omics data with rigor about which method preserves what (variance, local structure, manifold, transitions)… | GPTomics/ | 1.2k | 2 repos | ~4.8k | Automated safety check: Pass | MIT | 1 mo ago |
| 18 | Read, write, and create single-cell data objects using Seurat (R) and Scanpy (Python). | FreedomIntelligence/ | 3.1k | 1 repo | ~2k | Automated safety check: Pass | No licence | 2 mo ago |
| 19 | Quality control, filtering, and normalization for single-cell RNA-seq using Seurat (R) and Scanpy (Python). | FreedomIntelligence/ | 3.1k | 1 repo | ~2.4k | Automated safety check: Pass | No licence | 2 mo ago |
| 20 | Load spatial transcriptomics data from Visium, Xenium, MERFISH, Slide-seq, and other platforms using Squidpy and SpatialData. | FreedomIntelligence/ | 3.1k | 1 repo | ~2k | Automated safety check: Pass | No licence | 2 mo ago |
| 21 | Dimensionality reduction and clustering for single-cell RNA-seq using Seurat (R) and Scanpy (Python). | FreedomIntelligence/ | 3.1k | 1 repo | ~2k | Automated safety check: Pass | No licence | 2 mo ago |
| 22 | Find marker genes and annotate cell types in single-cell RNA-seq using Seurat (R) and Scanpy (Python). | FreedomIntelligence/ | 3.1k | 1 repo | ~2.2k | Automated safety check: Pass | No licence | 2 mo ago |
| 23 | Analyze multi-modal single-cell data (CITE-seq, Multiome, spatial). | FreedomIntelligence/ | 3.1k | 1 repo | ~1.7k | Automated safety check: Pass | No licence | 2 mo ago |
| 24 | Identify spatial domains and tissue regions in spatial transcriptomics data using Squidpy and Scanpy. | FreedomIntelligence/ | 3.1k | 1 repo | ~2.1k | Automated safety check: Pass | No licence | 2 mo ago |
| 25 | Quality control, filtering, normalization, and feature selection for spatial transcriptomics data. | FreedomIntelligence/ | 3.1k | 1 repo | ~2k | Automated safety check: Pass | No licence | 2 mo ago |
| 26 | Visualize spatial transcriptomics data using Squidpy and Scanpy. | FreedomIntelligence/ | 3.1k | 1 repo | ~2k | Automated safety check: Pass | No licence | 2 mo ago |
| 27 | Reads, inspects, and writes Flow Cytometry Standard (FCS) files from conventional, spectral, and mass cytometry (CyTOF), and parses FlowJo/Cytobank/Diva workspaces. | GPTomics/ | 1.2k | 1 repo | ~2.5k | Automated safety check: Pass | MIT | 1 mo ago |
| 28 | Dimensionality reduction and graph-based clustering for single-cell RNA-seq with Scanpy (Python) and Seurat (R). | GPTomics/ | 1.2k | 1 repo | ~3.5k | Automated safety check: Pass | MIT | 1 mo ago |
| 29 | Detect cluster marker genes and assign manual cell type labels in single-cell RNA-seq using Scanpy (Python) and Seurat (R). | GPTomics/ | 1.2k | 1 repo | ~3.4k | Automated safety check: Pass | MIT | 1 mo ago |
| 30 | Quality control, ambient-RNA handling, normalization, and feature selection for single-cell RNA-seq using Scanpy (Python) and Seurat (R). | GPTomics/ | 1.2k | 1 repo | ~5.1k | Automated safety check: Pass | MIT | 1 mo ago |
| 31 | Quality control, filtering, and normalization for spatial transcriptomics (Visium, Visium HD, Xenium, MERFISH/MERSCOPE, CosMx, Slide-seq) with Squidpy and Scanpy. | GPTomics/ | 1.2k | 1 repo | ~4.7k | Automated safety check: Pass | MIT | 1 mo ago |
| 32 | Plots spatial transcriptomics expression, clusters, and annotations on tissue using Squidpy and Scanpy. | GPTomics/ | 1.2k | 1 repo | ~3.6k | Automated safety check: Pass | MIT | 1 mo ago |
| 33 | Orchestrates the end-to-end spatial transcriptomics pipeline from Space Ranger / vendor output to spatial domains and statistics, branching FIRST on platform class (imaging in-situ… | GPTomics/ | 1.2k | 1 repo | ~4.2k | Automated safety check: Pass | MIT | 1 mo ago |
| 34 | 34.Scvelo RNA velocity analysis with scVelo. An agent skill from lamm-mit/scienceclaw. | lamm-mit/ | 244 | 4 repos | ~524 | Automated safety check: Pass | BSD-3-Clause | 1 mo ago |
| 35 | Open-source FAIR biology data framework. An agent skill from jaechang-hits/SciAgent-Skills. | jaechang-hits/ | 371 | 2 repos | ~4k | Automated safety check: Pass | Apache-2.0 | 10 days ago |
| 36 | 36.Biopython Primary retained Python toolkit for molecular biology sequence work. | foryourhealth111-pixel/ | 3.6k | — | ~3.5k | Automated safety check: Pass | Apache-2.0 | 1 mo ago |
| 37 | 37.Scanpy Standard single-cell RNA-seq analysis pipeline. An agent skill from aipoch/medical-research-skills. | aipoch/ | 2k | — | ~3.9k | Automated safety check: Pass | MIT | 22 days ago |
| 38 | Normalizes and transforms RNA-seq count matrices for DE, visualization, clustering, and ML. | GPTomics/ | 1.2k | 1 repo | ~6.2k | Automated safety check: Pass | MIT | 1 mo ago |
| 39 | Stores and operates on sparse expression matrices for single-cell and large bulk RNA-seq, covering dgCMatrix/dgRMatrix/dgTMatrix when-each-is-fast, the dgCMatrix (CSC, R) <- CSR (Python) implicit… | GPTomics/ | 1.2k | 1 repo | ~5.6k | Automated safety check: Pass | MIT | 1 mo ago |
| 40 | scRNA-seq with Scanpy: QC, normalization, HVG selection, PCA, neighborhood graph, UMAP/t-SNE, Leiden clustering, markers, cell annotation, trajectory inference. | jaechang-hits/ | 371 | 1 repo | ~4.7k | Automated safety check: Pass | CC-BY-4.0 | 10 days ago |
| 41 | 41.Geniml Machine learning toolkit for genomic interval (BED) data; use it when you need to tokenize BED collections and train embeddings for regions/cells/labels, build consensus peak universes, or run… | aipoch/ | 2k | — | ~1.9k | Automated safety check: Pass | MIT | 22 days ago |
| 42 | Harmony batch correction for scRNA-seq and other omics. An agent skill from jaechang-hits/SciAgent-Skills. | jaechang-hits/ | 371 | 2 repos | ~5.6k | Automated safety check: Pass | MIT | 10 days ago |
| 43 | Multi-modal single-cell analysis with muon/MuData. An agent skill from jaechang-hits/SciAgent-Skills. | jaechang-hits/ | 371 | 2 repos | ~8.1k | Automated safety check: Pass | BSD-3-Clause | 10 days ago |
| 44 | 44.Sc De Load when finding marker genes per cluster or comparing condition expression in single-cell RNA-seq. | TianGzlab/ | 161 | — | ~2.3k | Automated safety check: Pass | Apache-2.0 | yesterday |
| 45 | 45.Sc Markers Load when ranking cluster-level marker genes from a clustered single-cell AnnData via Scanpy Wilcoxon / t-test / logreg or COSG specificity. | TianGzlab/ | 161 | — | ~2.2k | Automated safety check: Pass | Apache-2.0 | yesterday |
| 46 | Load when computing per-cell pathway / gene-set scores on a normalised scRNA AnnData via AUCell (R or Python) or Scanpy scoregenes. | TianGzlab/ | 161 | — | ~2.2k | Automated safety check: Pass | Apache-2.0 | yesterday |
| 47 | Load when normalising QC'd scRNA into a PCA-ready AnnData via scanpy / Seurat / SCTransform / Pearson residuals. | TianGzlab/ | 161 | — | ~2.8k | Automated safety check: Pass | Apache-2.0 | yesterday |
| 48 | Local Scanpy pipeline for single-cell RNA-seq QC, clustering, marker discovery, and optional two-group differential expression from raw-count .h5ad. | FreedomIntelligence/ | 3.1k | — | ~1.7k | Automated safety check: Pass | MIT | 2 mo ago |