Single Cell Rna Qc
FreedomIntelligence/OpenClaw-Medical-Skills
Performs quality control on single-cell RNA-seq data (.h5ad or .h5 files) using scverse best practices with MAD-based filtering and comprehensive visualizations.
Local Scanpy pipeline for single-cell RNA-seq QC, optional doublet detection, clustering, marker discovery, optional CellTypist annotation, optional latent downstream mode from…
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a claude-codeProject install by default; add -g for ~/.claude/skills/.
$ gh skill install ClawBio/ClawBio scrna-orchestrator --agent claude-codeProject scope by default; add --scope user for a personal install. Needs GitHub CLI 2.90.0 or later (public preview).
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .claude/skills && cp -r skills-src/skills/scrna-orchestrator .claude/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.claude/skills/ instead of .claude/skills for a personal install. The folder must contain SKILL.md.
Claude Code skills documentation · loads skills from .claude/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .claude/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.Claude Code copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$skill-installer install https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestratorType this inside Codex. $skill-installer <name> installs a curated skill from openai/skills. The installer writes to $CODEX_HOME/skills (default ~/.codex/skills). Restart Codex if the skill does not show up.
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a codexProject install goes to .agents/skills/; add -g for ~/.codex/skills/.
$ gh skill install ClawBio/ClawBio scrna-orchestrator --agent codexProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .agents/skills && cp -r skills-src/skills/scrna-orchestrator .agents/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.agents/skills/ instead of .agents/skills for a personal install.
Codex skills documentation · loads skills from .agents/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .agents/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.Codex copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a cursorProject install goes to .agents/skills/; add -g for ~/.cursor/skills/.
$ gh skill install ClawBio/ClawBio scrna-orchestrator --agent cursorProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .cursor/skills && cp -r skills-src/skills/scrna-orchestrator .cursor/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.cursor/skills/ instead of .cursor/skills for a personal install.
Cursor skills documentation · loads skills from .cursor/skills/, .agents/skills/, .claude/skills/, .codex/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .cursor/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.Cursor copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gemini skills install https://github.com/ClawBio/ClawBio.git --path skills/scrna-orchestrator--scope user (default) or --scope workspace; --path is the subfolder of the repo that holds the skill; --consent skips the security confirmation prompt.
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a gemini-cliProject install goes to .agents/skills/; add -g for ~/.gemini/skills/.
$ gh skill install ClawBio/ClawBio scrna-orchestrator --agent gemini-cliProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .gemini/skills && cp -r skills-src/skills/scrna-orchestrator .gemini/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.gemini/skills/ instead of .gemini/skills for a personal install, then run /skills reload.
Gemini CLI skills documentation · loads skills from .gemini/skills/, .agents/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .gemini/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.Gemini CLI copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gh skill install ClawBio/ClawBio scrna-orchestratorInstalls for Copilot at project scope by default; add --scope user for a personal install. Preview a skill first with gh skill preview. Needs GitHub CLI 2.90.0 or later (public preview).
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a github-copilotProject install goes to .agents/skills/; add -g for ~/.copilot/skills/.
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .github/skills && cp -r skills-src/skills/scrna-orchestrator .github/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.copilot/skills/ instead of .github/skills for a personal install. Commit .github/skills so cloud agent and code review can use it.
GitHub Copilot skills documentation · loads skills from .github/skills/, .claude/skills/, .agents/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .github/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.GitHub Copilot copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a opencodeOpenCode documents no install command of its own. Project install goes to .agents/skills/; add -g for ~/.config/opencode/skills/.
$ gh skill install ClawBio/ClawBio scrna-orchestrator --agent opencodeProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/ClawBio/ClawBio.git skills-src && mkdir -p .opencode/skills && cp -r skills-src/skills/scrna-orchestrator .opencode/skills/scrna-orchestrator && rm -rf skills-srcUse ~/.config/opencode/skills/ instead of .opencode/skills for a personal install.
OpenCode skills documentation · loads skills from .opencode/skills/, .claude/skills/, .agents/skills/
Install the "scrna-orchestrator" agent skill from https://github.com/ClawBio/ClawBio/tree/main/skills/scrna-orchestrator into .opencode/skills/scrna-orchestrator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "scrna-orchestrator", then confirm the skill loads.OpenCode copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
scrna-orchestratorLocal Scanpy pipeline for single-cell RNA-seq QC, optional doublet detection, clustering, marker discovery, optional CellTypist annotation, optional latent downstream mode from…
Scrna Orchestrator is an agent skill from ClawBio/ClawBio. Local Scanpy pipeline for single-cell RNA-seq QC, optional doublet detection, clustering, marker discovery, optional CellTypist annotation, optional latent downstream mode from integrated.h5ad/Xscvi, and optional dataset-level plus within-cluster contrastive marker analysis from raw-count .h5ad or 10x Matrix Market input.
Its SKILL.md is about 2.9k tokens, which your agent loads only when the skill is triggered. The skill folder holds 3 other files (for example `scrna_orchestrator.py` and `tests/test_scrna_orchestrator.py`).
It sits in Research & Science, covering Bioinformatics. It works with Scanpy. The repository describes itself as: 🦖 ClawBio - The first bioinformatics-native AI agent skill library. Local-first. Reproducible. Open. Free. The licence is MIT.
9 steps, taken from the first numbered list in SKILL.md.
Read from SKILL.md and the folder at commit dece754. It shows what the files ask for, not the result of running them.
Pre-approves nothing: there is no allowed-tools line, so your agent's usual permission prompts apply.
From allowed-tools in the SKILL.md frontmatter.
Ships script files (Python), which the agent can run.
Shell commands in SKILL.md call:
pythonFrom the folder's file list and the shell code blocks in SKILL.md.
Links to these hosts (documentation or services it may open):
scanpy.readthedocs.ioanndata.readthedocs.ionature.comgenomebiology.biomedcentral.comcelltypist.orgFrom URLs in SKILL.md, links to its own repository left out.
Names no API keys, tokens, secrets or passwords.
From names ending in _API_KEY, _TOKEN, _SECRET, _KEY or _PASSWORD in SKILL.md.
Scrna Orchestrator loads about 2.9k tokens when it runs. Until then it costs about 86 tokens; SKILL.md has 846 words of instructions outside code blocks.
Estimates: characters ÷ 4, the usual rule of thumb; real counts depend on the model's tokenizer. Scripts and assets cost tokens only if the agent reads them.
The automated check found no risky patterns in SKILL.md.
Automated static check — not a guarantee. Review scripts before installing. It scans the text of SKILL.md for risky patterns (piping downloads into a shell, reading credential files, hidden Unicode, destructive commands); files beside SKILL.md are not scanned.
The full file from ClawBio/ClawBio at commit dece754, republished under its MIT licence (© ClawBio). 846 words, ~2,869 tokens.
.claude/skills/scrna-orchestrator/SKILL.md (or your agent's skills folder). This skill also uses 2 other files; get the full folder from GitHub.You are scRNA Orchestrator, a specialised ClawBio agent for local single-cell RNA-seq analysis with Scanpy.
Single-cell workflows are easy to misconfigure and hard to reproduce when run ad hoc.
report.md, figures, tables, structured metadata, and a reproducibility bundle, whether the graph is built from PCA or X_scvi.log1p, and HVG selection.obs column.| Format | Extension | Required Fields | Example |
|---|---|---|---|
| AnnData raw counts or latent downstream artifact | .h5ad | Raw count matrix in X or recoverable raw counts in layers["counts"]; optional latent rep in obsm["X_scvi"]; cell metadata in obs; gene metadata in var | pbmc_raw.h5ad, integrated.h5ad |
| 10x Matrix Market | directory, .mtx, .mtx.gz | matrix.mtx(.gz) plus matching barcodes.tsv(.gz) and features.tsv(.gz) or genes.tsv(.gz) | filtered_feature_bc_matrix/ |
| Demo mode | n/a | none | python clawbio.py run scrna --demo |
Notes:
.h5ad inputs are rejected unless they are a recoverable latent downstream artifact with raw counts preserved in layers["counts"].matrix.mtx(.gz).pbmc3k_processed-style inputs are out of scope for this skill.When the user asks for scRNA QC/clustering/markers/annotation/contrastive markers:
.h5ad or 10x Matrix Market input (or --demo), and reject processed-like matrices.log1p, select HVGs, and build the graph from PCA or a latent rep such as X_scvi.leiden, Wilcoxon).--contrast-groupby is provided.report.md, result.json, tables, figures, and reproducibility bundle.# Standard usage
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <input.h5ad> --output <report_dir>
# 10x Matrix Market directory
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <filtered_feature_bc_matrix_dir> --output <report_dir>
# Direct matrix.mtx(.gz) path
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <matrix.mtx.gz> --output <report_dir>
# Demo mode
python skills/scrna-orchestrator/scrna_orchestrator.py \
--demo --output <report_dir>
# Optional doublet detection
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <input.h5ad> --output <report_dir> \
--doublet-method scrublet
# Optional CellTypist annotation
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <input.h5ad> --output <report_dir> \
--annotate celltypist --annotation-model Immune_All_Low
# Optional dataset-level pairwise contrasts
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <input.h5ad> --output <report_dir> \
--contrast-groupby <obs_column> --contrast-scope dataset
# Optional dataset-level + within-cluster contrasts together
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <input.h5ad> --output <report_dir> \
--contrast-groupby <obs_column> --contrast-scope both \
--contrast-clusterby leiden
# Optional latent downstream mode
python skills/scrna-orchestrator/scrna_orchestrator.py \
--input <integrated.h5ad> --output <report_dir> \
--use-rep X_scvi
# Via ClawBio runner
python clawbio.py run scrna --input <input.h5ad> --output <report_dir>
python clawbio.py run scrna --input <filtered_feature_bc_matrix_dir> --output <report_dir>
python clawbio.py run scrna --demopython clawbio.py run scrna --demo
python clawbio.py run scrna --demo --doublet-method scrubletExpected output:
report.md with QC, clustering, markers, and optional annotation/contrast summariesqc_violin.png, umap_leiden.png, marker_dotplot.png)n_genes_by_counts, total_counts, pct_counts_mt)min_genes, min_cells, max_mt_pctscanpy.pp.scrublet on QC-filtered raw counts1e4log1pflavor="seurat")max_value=10) on the HVG branchscanpy.tl.rank_genes_groups(groupby="leiden", method="wilcoxon", pts=True)--contrast-groupby, run scanpy.tl.rank_genes_groups(..., groups=[group1], reference=group2, method="wilcoxon", pts=True)--contrast-clusterby and every unordered pair of observed groups in --contrast-groupby, run the same Wilcoxon contrast on the cluster subsetoutput_directory/
├── report.md
├── result.json
├── figures/
│ ├── qc_violin.png
│ ├── umap_leiden.png
│ └── marker_dotplot.png
├── tables/
│ ├── cluster_summary.csv
│ ├── markers_top.csv
│ ├── markers_top.tsv
│ ├── doublet_summary.csv # only when doublet detection is enabled
│ ├── cluster_annotations.csv # only when annotation is enabled
│ ├── contrastive_markers_full.csv # only when dataset-level contrasts are enabled
│ ├── contrastive_markers_top.csv # only when dataset-level contrasts are enabled
│ ├── within_cluster_contrastive_markers_full.csv # only when within-cluster contrasts are enabled
│ └── within_cluster_contrastive_markers_top.csv # only when within-cluster contrasts are enabled
└── reproducibility/
├── commands.sh
├── environment.yml
└── checksums.sha256Required:
scanpy >= 1.10anndata >= 0.10scipynumpy, pandas, matplotlib, leidenalg, python-igraphOptional:
scrublet for --doublet-method scrubletcelltypist for --annotate celltypistOut of scope:
scvi-tools / scANVITrigger conditions:
.h5ad, .mtx, or .mtx.gzCurrent limitations:
.h5ad and 10x Matrix Market onlyMVP implemented -- supports .h5ad and 10x Matrix Market input, PBMC3k-first demo data (fallback to synthetic on failure), opt-in Scrublet doublet detection, opt-in local CellTypist annotation, opt-in latent downstream mode from integrated.h5ad, and opt-in dataset-level plus within-cluster pairwise contrastive markers.
© ClawBio, MIT. Rendered from Markdown: HTML in the file is shown as text, images as links, and headings moved down two levels. Raw file
SKILL.md and 2 other files in skills/scrna-orchestrator of ClawBio/ClawBio.
Open the folder on GitHubat commit dece754
We found 1 copy of this SKILL.md (exact, near-identical or edited) in other folders, from 1 other GitHub owner. This page covers the copy in ClawBio/ClawBio, which our catalogue first saw on October 7, 2026.
Scrna Orchestrator next to the 5 skills that share the most tags, products or categories with it. Stars are the repository's; “used in” counts other GitHub owners with a copy.
| Skill | Stars | Used in | Tokens | Auto-check | Licence | Repo updated |
|---|---|---|---|---|---|---|
| Scrna Orchestrator this skillClawBio/ClawBio | 1.2k | 1 repos | ~2.9k | Automated safety check: Pass | MIT | |
| Single Cell Rna QcFreedomIntelligence/OpenClaw-Medical-Skills | 3.1k | 2 repos | ~2k | Automated safety check: Pass | Apache-2.0 | |
| Scanpy Single-Cell Analysisdavila7/claude-code-templates | 33k | 15 repos | ~2.8k | Automated safety check: Pass | MIT | |
| Single Cell Rna AnalysisPKU-YuanGroup/OpenAI4S | 622 | — | ~1.3k | Automated safety check: Pass | MIT | |
| Anndatadavila7/claude-code-templates | 33k | 11 repos | ~2.5k | Automated safety check: Pass | MIT | |
| Cellxgene Censusdavila7/claude-code-templates | 33k | 11 repos | ~3.8k | Automated safety check: Pass | MIT |
FreedomIntelligence/OpenClaw-Medical-Skills
Performs quality control on single-cell RNA-seq data (.h5ad or .h5 files) using scverse best practices with MAD-based filtering and comprehensive visualizations.
davila7/claude-code-templates
Walks through single-cell RNA-seq analysis with Scanpy: loading .h5ad and 10X data, QC, normalization, PCA and UMAP, Leiden clustering, marker genes and cell type annotation.
PKU-YuanGroup/OpenAI4S
Reproducible Scanpy workflow for human or mouse 10x scRNA-seq and snRNA-seq count matrices: single-sample descriptive QC, clustering and annotation, or comparative donor-aware pseudobulk DE and Milo…
davila7/claude-code-templates
This skill should be used when working with annotated data matrices in Python, particularly for single-cell genomics analysis, managing experimental measurements with metadata, or handling…
davila7/claude-code-templates
Query CZ CELLxGENE Census (61M+ cells). An agent skill from davila7/claude-code-templates.
K-Dense-AI/scientific-agent-skills
Prepares bulk RNA-seq FASTQ, Salmon, STAR or featureCounts output for gene-level differential expression.
ClawBio/ClawBio
Fetch a region of cis-eQTL summary statistics from EBI eQTL Catalogue v7+ via tabix-on-FTP.
ClawBio/ClawBio
Query TCGA tumor biology through the ucscxenatoolspy API. An agent skill from ClawBio/ClawBio.
ClawBio/ClawBio
Fetch a region of GWAS summary statistics from the NHGRI-EBI GWAS Catalog harmonised collection via tabix-on-FTP.
ClawBio/ClawBio
Population genetics of pre-aligned DNA sequences or multi-sample VCFs using selected DnaSP 6 methods.
ClawBio/ClawBio
Compute pairwise r² between a lead variant and every variant in a window using the 1000 Genomes Phase 3 GRCh38 reference panel, ancestry-stratified.
ClawBio/ClawBio
Download genomes, genes, virus sequences, and taxonomy data from NCBI using the datasets and dataformat CLI tools.
Works with
Categories
Local Scanpy pipeline for single-cell RNA-seq QC, optional doublet detection, clustering, marker discovery, optional CellTypist annotation, optional latent downstream mode from…. Scrna Orchestrator is an agent skill from ClawBio/ClawBio.h5ad or 10x Matrix Market input.
Scrna Orchestrator fits situations like: tasks that involve Bioinformatics.
Run `npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a claude-code`. Or copy the skill folder (skills/scrna-orchestrator in ClawBio/ClawBio) into .claude/skills/scrna-orchestrator in your project. Claude Code loads it when a task matches its description.
Run `npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a codex`. Or copy the skill folder (skills/scrna-orchestrator in ClawBio/ClawBio) into .agents/skills/scrna-orchestrator in your project. Codex loads it when a task matches its description.
Cursor, Gemini CLI, GitHub Copilot and OpenCode also load SKILL.md folders. With the skills CLI, run `npx skills add ClawBio/ClawBio --skill scrna-orchestrator -a cursor` (or -a gemini-cli, github-copilot or opencode for the others). To copy it by hand, put the folder in .cursor/skills/scrna-orchestrator, .gemini/skills/scrna-orchestrator, .github/skills/scrna-orchestrator and .opencode/skills/scrna-orchestrator in your project.
Going by SKILL.md and its folder, Scrna Orchestrator needs Python for the scripts in its folder and the command-line tools its instructions call (python). Our summary lists: Python 3.
SKILL.md names 5 domains. As links in the text: scanpy.readthedocs.io, anndata.readthedocs.io, nature.com, genomebiology.biomedcentral.com and celltypist.org. This is read from the text; nothing was executed.
Our automated static check of SKILL.md found no risky patterns, such as piping downloads into a shell, reading credential files or hidden Unicode. It is not a guarantee. Review the folder before installing.
Scrna Orchestrator is published under the MIT licence (declared in SKILL.md). It allows redistribution, so the full SKILL.md is shown on this page.
About 2.9k tokens (SKILL.md is roughly 11k characters). Agents keep only the skill's name and description in context until a task matches; then they load SKILL.md in full.
Skills that share tags, products or a category with Scrna Orchestrator: Single Cell Rna Qc (FreedomIntelligence/OpenClaw-Medical-Skills, 3.1k stars), Scanpy Single-Cell Analysis (davila7/claude-code-templates, 33k stars), Single Cell Rna Analysis (PKU-YuanGroup/OpenAI4S, 622 stars) and Anndata (davila7/claude-code-templates, 33k stars). The comparison table on this page puts their stars, adoption, token cost, safety result and licence side by side.
ClawBio (a GitHub organization) maintains it in ClawBio/ClawBio, which has 1,155 GitHub stars. The repository holds 104 skills in this directory. The repository was last updated on October 9, 2026.
Source: ClawBio/ClawBio on GitHub. Facts on this page come from the repository at the commit we read; the author's words are quoted as theirs.