Buffer
LeoYeAI/openclaw-master-skills
Buffer API integration with managed authentication. An agent skill from LeoYeAI/openclaw-master-skills.
Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry.
MITAuto-check passed
Install Buffer Calculator
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a claude-codeProject install by default; add -g for ~/.claude/skills/.
$ gh skill install aipoch/medical-research-skills buffer-calculator --agent claude-codeProject scope by default; add --scope user for a personal install. Needs GitHub CLI 2.90.0 or later (public preview).
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .claude/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .claude/skills/buffer-calculator && rm -rf skills-srcUse ~/.claude/skills/ instead of .claude/skills for a personal install. The folder must contain SKILL.md.
Claude Code skills documentation · loads skills from .claude/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .claude/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.Claude Code copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$skill-installer install https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculatorType this inside Codex. $skill-installer <name> installs a curated skill from openai/skills. The installer writes to $CODEX_HOME/skills (default ~/.codex/skills). Restart Codex if the skill does not show up.
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a codexProject install goes to .agents/skills/; add -g for ~/.codex/skills/.
$ gh skill install aipoch/medical-research-skills buffer-calculator --agent codexProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .agents/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .agents/skills/buffer-calculator && rm -rf skills-srcUse ~/.agents/skills/ instead of .agents/skills for a personal install.
Codex skills documentation · loads skills from .agents/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .agents/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.Codex copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a cursorProject install goes to .agents/skills/; add -g for ~/.cursor/skills/.
$ gh skill install aipoch/medical-research-skills buffer-calculator --agent cursorProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .cursor/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .cursor/skills/buffer-calculator && rm -rf skills-srcUse ~/.cursor/skills/ instead of .cursor/skills for a personal install.
Cursor skills documentation · loads skills from .cursor/skills/, .agents/skills/, .claude/skills/, .codex/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .cursor/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.Cursor copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gemini skills install https://github.com/aipoch/medical-research-skills.git --path scientific-skills/Other/buffer-calculator--scope user (default) or --scope workspace; --path is the subfolder of the repo that holds the skill; --consent skips the security confirmation prompt.
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a gemini-cliProject install goes to .agents/skills/; add -g for ~/.gemini/skills/.
$ gh skill install aipoch/medical-research-skills buffer-calculator --agent gemini-cliProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .gemini/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .gemini/skills/buffer-calculator && rm -rf skills-srcUse ~/.gemini/skills/ instead of .gemini/skills for a personal install, then run /skills reload.
Gemini CLI skills documentation · loads skills from .gemini/skills/, .agents/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .gemini/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.Gemini CLI copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gh skill install aipoch/medical-research-skills buffer-calculatorInstalls for Copilot at project scope by default; add --scope user for a personal install. Preview a skill first with gh skill preview. Needs GitHub CLI 2.90.0 or later (public preview).
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a github-copilotProject install goes to .agents/skills/; add -g for ~/.copilot/skills/.
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .github/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .github/skills/buffer-calculator && rm -rf skills-srcUse ~/.copilot/skills/ instead of .github/skills for a personal install. Commit .github/skills so cloud agent and code review can use it.
GitHub Copilot skills documentation · loads skills from .github/skills/, .claude/skills/, .agents/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .github/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.GitHub Copilot copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add aipoch/medical-research-skills --skill buffer-calculator -a opencodeOpenCode documents no install command of its own. Project install goes to .agents/skills/; add -g for ~/.config/opencode/skills/.
$ gh skill install aipoch/medical-research-skills buffer-calculator --agent opencodeProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/aipoch/medical-research-skills.git skills-src && mkdir -p .opencode/skills && cp -r skills-src/scientific-skills/Other/buffer-calculator .opencode/skills/buffer-calculator && rm -rf skills-srcUse ~/.config/opencode/skills/ instead of .opencode/skills for a personal install.
OpenCode skills documentation · loads skills from .opencode/skills/, .claude/skills/, .agents/skills/
Install the "buffer-calculator" agent skill from https://github.com/aipoch/medical-research-skills/tree/main/scientific-skills/Other/buffer-calculator into .opencode/skills/buffer-calculator/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "buffer-calculator", then confirm the skill loads.OpenCode copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
Facts
- Skill name
buffer-calculator- GitHub stars
- 2k
- Token cost
- ~1.8k tokens
- SKILL.md length
- 709 words
- Files
- 3 (incl. scripts)
- Skills in repo
- 567
- Repo updated
- First seen
- Licence
- MIT
At a glance
Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry.
- Works in 3 steps: Predefined Buffer Library → Mass Calculations → Stock Solution Scaling
- SKILL.md covers Input Validation, Quick Check, Workflow and Core Capabilities, plus 8 more sections
- Runs Python scripts from its folder; calls python
What it does
Buffer Calculator is an agent skill from aipoch/medical-research-skills. Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry. Supports PBS, RIPA, and TAE with concentration scaling, stock solution preparation, pH adjustment guidance, and step-by-step protocols.
Its SKILL.md is about 1.8k tokens, which your agent loads only when the skill is triggered. The skill folder holds 3 other files, including scripts (for example `buffer-calculator_audit_result_v2.json` and `scripts/main.py`).
The repository describes itself as: Hundreds of agent skills for medical research, including protocol design, data analysis, evidence insights, and academic writing. The licence is MIT.
Example prompts
- “/buffer-calculator”
Requirements
- Python 3
Workflow steps
3 steps, taken from the step headings in SKILL.md.
What it can do on your machine
Read from SKILL.md and the folder at commit 686e09d. It shows what the files ask for, not the result of running them.
Tool permissions
Pre-approves nothing: there is no allowed-tools line, so your agent's usual permission prompts apply.
From allowed-tools in the SKILL.md frontmatter.
Runs code
Ships 1 file in scripts/ (Python), which the agent can run.
Shell commands in SKILL.md call:
python
From the folder's file list and the shell code blocks in SKILL.md.
Network
Links to these hosts (documentation or services it may open):
cshprotocols.orgthermofisher.com
From URLs in SKILL.md, links to its own repository left out.
Credentials
Names no API keys, tokens, secrets or passwords.
From names ending in _API_KEY, _TOKEN, _SECRET, _KEY or _PASSWORD in SKILL.md.
Context cost
Buffer Calculator loads about 1.8k tokens when it runs. Until then it costs about 67 tokens; SKILL.md has 709 words of instructions outside code blocks.
Estimates: characters ÷ 4, the usual rule of thumb; real counts depend on the model's tokenizer. Scripts and assets cost tokens only if the agent reads them.
Safety
Auto-check passed
The automated check found no risky patterns in SKILL.md.
Automated static check — not a guarantee. Review scripts before installing. It scans the text of SKILL.md for risky patterns (piping downloads into a shell, reading credential files, hidden Unicode, destructive commands); the scripts in this folder are not scanned.
SKILL.md
The full file from aipoch/medical-research-skills at commit 686e09d, republished under its MIT licence (© aipoch). 709 words, ~1,772 tokens.
Download SKILL.mdSave it as
.claude/skills/buffer-calculator/SKILL.md (or your agent's skills folder). This skill also uses 2 other files; get the full folder from GitHub.- name
- buffer-calculator
- description
- Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry. Supports PBS, RIPA, and TAE with concentration scaling, stock solution preparation, pH adjustment guidance, and step-by-step protocols.
- license
- MIT
- author
- AIPOCH
Buffer Calculator
Calculate precise buffer formulations with accurate mass and volume measurements for molecular biology, biochemistry, and cell culture applications. Supports predefined common buffers and customizable calculations with pH adjustment guidance.
Key Capabilities:
- Predefined Buffer Library: PBS, RIPA, TAE with accurate molecular weights
- Precise Mass Calculations: Component masses to milligram precision
- Volume-Based Components: Handle liquid components (detergents, acids)
- Concentration Scaling: Scale from stock solutions (10X, 20X) to working concentrations
- Step-by-Step Protocols: Detailed preparation instructions with safety notes
Input Validation
This skill accepts: a buffer type (PBS, RIPA, TAE), final volume in mL, and optional concentration multiplier (default 1X).
If the request does not involve calculating a laboratory buffer recipe — for example, asking to design a drug formulation, interpret pH meter readings, or perform chemical synthesis — do not proceed. Instead respond:
"Buffer Calculator is designed to calculate precise buffer recipes for molecular biology and biochemistry. Please provide a buffer type (PBS, RIPA, or TAE) and target volume. For other formulation tasks, use a more appropriate tool."
Quick Check
python -m py_compile scripts/main.py
python scripts/main.py --helpWorkflow
- Confirm buffer type, final volume, and concentration multiplier.
- Validate that the buffer type is in the supported library; stop if unsupported without guessing.
- Run the calculation script or apply the documented formula path.
- Return a structured result separating assumptions, deliverables, risks, and unresolved items.
- If execution fails or inputs are incomplete, switch to the fallback path and state exactly what blocked full completion.
Fallback: If buffer type is missing or unrecognized, respond: "Buffer type not specified or not in library. Available buffers: PBS, RIPA, TAE. Use --list to see all options. Cannot calculate without a valid buffer type."
Core Capabilities
1. Predefined Buffer Library
from scripts.main import BufferCalculator
calc = BufferCalculator()
# List available buffers
for buf in calc.BUFFER_RECIPES.keys():
print(f" {buf}: pH {calc.BUFFER_RECIPES[buf].get('pH', 'N/A')}")| Buffer | Application | pH | Key Components |
|---|---|---|---|
| PBS | Cell washing, immunostaining | 7.4 | NaCl, KCl, Phosphates |
| RIPA | Cell lysis, protein extraction | 7.4 | Tris, NaCl, Detergents |
| TAE | DNA electrophoresis | ~8.0 | Tris, Acetate, EDTA |
| HEPES | Cell culture, pH-sensitive assays | 7.0–7.6 | HEPES, NaCl |
| Tris-HCl (pH 7.4) | Protein buffers, Western blot | 7.4 | Tris, HCl |
| Tris-HCl (pH 8.0) | DNA/RNA work, enzyme reactions | 8.0 | Tris, HCl |
| MOPS | RNA electrophoresis, cell culture | 7.0–7.5 | MOPS, NaCl |
2. Mass Calculations
result = calc.calculate("PBS", final_volume_ml=500, concentration_x=1.0)
for comp in result['components']:
if 'amount_mg' in comp:
print(f"{comp['component']}: {comp['amount_mg']:.2f} mg")Formula: mass (mg) = concentration (mM) × volume (mL) × MW (g/mol) / 1000
3. Stock Solution Scaling
# 10X PBS stock (500 mL)
stock_result = calc.calculate("PBS", final_volume_ml=500, concentration_x=10.0)| Concentration | Storage Stability | Use Case |
|---|---|---|
| 1X | 1–2 weeks at 4°C | Immediate use |
| 10X | 3–6 months at 4°C | Regular daily use |
| 20X–50X | 6–12 months frozen | Long-term storage |
CLI Usage
# Calculate PBS buffer (1X, 500 mL)
python scripts/main.py PBS --volume 500
# Calculate 10X PBS
python scripts/main.py PBS --volume 500 --concentration 10
# List all available buffers
python scripts/main.py --listParameters
| Parameter | Type | Required | Description |
|---|---|---|---|
buffer | string | Yes | Buffer type (PBS, RIPA, TAE) |
--volume, -v | float | No | Final volume in mL |
--concentration, -c | float | No (default 1.0) | Concentration multiplier (X) |
--list, -l | flag | No | List available buffers |
Show full SKILL.md (283 more words)Show less
Output Requirements
Every final response must make these explicit:
- Objective or requested deliverable
- Inputs used (buffer type, volume, concentration) and assumptions introduced
- Calculation formula applied
- Core result: component masses/volumes with units
- Constraints and risks (verify MW for hydrates; check pH after preparation)
- Unresolved items and next-step checks (pH verification, sterile filtration if needed)
Error Handling
- If buffer type is missing or unrecognized, list available options and request clarification. If the user provides component names, concentrations, and molecular weights, offer to calculate a custom recipe using the formula:
mass (mg) = concentration (mM) × volume (mL) × MW (g/mol) / 1000. - If volume is not provided, use a sensible default (500 mL) and state the assumption explicitly.
- If
scripts/main.pyfails, report the failure point and provide manual calculation fallback using the formula above. - Do not fabricate molecular weights or component amounts.
Quick Verification
Expected output for PBS 1X 500 mL:
- NaCl: 4,000 mg (137 mM × 500 mL × 58.44 g/mol / 1000)
- KCl: 100 mg (2.7 mM × 500 mL × 74.55 g/mol / 1000)
- Na₂HPO₄: 720 mg (10.1 mM × 500 mL × 141.96 g/mol / 1000)
- KH₂PO₄: 120 mg (1.76 mM × 500 mL × 136.09 g/mol / 1000)
Common Pitfalls
- Confusing mM and M: 1000-fold concentration error — always verify units
- Wrong molecular weight: Account for hydrates (e.g., Na₂HPO₄·7H₂O vs anhydrous)
- Adding water to acid: Always add acid to water, never reverse
- Incomplete dissolution: Dissolve each component completely before adding next
- pH drift during storage: Check pH before each use for critical applications
Molecular Weight Reference
| Compound | Formula | MW (g/mol) |
|---|---|---|
| NaCl | NaCl | 58.44 |
| KCl | KCl | 74.55 |
| Tris base | C₄H₁₁NO₃ | 121.14 |
| EDTA (disodium) | C₁₀H₁₄N₂Na₂O₈·2H₂O | 372.24 |
| Na₂HPO₄ (anhydrous) | Na₂HPO₄ | 141.96 |
| KH₂PO₄ | KH₂PO₄ | 136.09 |
References
- Cold Spring Harbor Protocols: https://cshprotocols.org
- Thermo Fisher Buffer Reference: https://www.thermofisher.com/buffers
→ Full troubleshooting: references/troubleshooting.md (if available)
© aipoch, MIT. Rendered from Markdown: HTML in the file is shown as text, images as links, and headings moved down two levels. Raw file
Files
SKILL.md and 2 other files (scripts) in scientific-skills/Other/buffer-calculator of aipoch/medical-research-skills.
- SKILL.md
- buffer-calculator_audit_result_v2.json
- scripts/main.py
Open the folder on GitHubat commit 686e09d
Compare with similar skills
Buffer Calculator next to the 5 skills that share the most tags, products or categories with it. Stars are the repository's; “used in” counts other GitHub owners with a copy.
| Skill | Stars | Used in | Tokens | Auto-check | Licence | Repo updated |
|---|---|---|---|---|---|---|
| Buffer Calculator this skillaipoch/medical-research-skills | 2k | — | ~1.8k | Automated safety check: Pass | MIT | |
| BufferLeoYeAI/openclaw-master-skills | 2.2k | — | ~7.5k | Automated safety check: Pass | MIT | |
| Measure Before You Fixgarrytan/gbrain | 31k | — | ~2.5k | Automated safety check: Pass | MIT | |
| Eol Resistor Calculatorsickn33/agentic-awesome-skills | 47k | 1 repos | ~3.5k | Automated safety check: Pass | MIT | |
| Material Density Volume CalculationInternScience/scp | 169 | 1 repos | ~497 | Automated safety check: Pass | MIT | |
| Mixed Precisionaiming-lab/AutoResearchClaw | 15k | — | ~275 | Automated safety check: Pass | MIT |
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Questions about Buffer Calculator
What does Buffer Calculator do?
Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry. Buffer Calculator is an agent skill from aipoch/medical-research-skills. Calculate precise buffer recipes with accurate mass and volume measurements for molecular biology and biochemistry.
How do I install Buffer Calculator in Claude Code?
Run `npx skills add aipoch/medical-research-skills --skill buffer-calculator -a claude-code`. Or copy the skill folder (scientific-skills/Other/buffer-calculator in aipoch/medical-research-skills) into .claude/skills/buffer-calculator in your project. Claude Code loads it when a task matches its description.
How do I install Buffer Calculator in Codex?
Run `npx skills add aipoch/medical-research-skills --skill buffer-calculator -a codex`. Or copy the skill folder (scientific-skills/Other/buffer-calculator in aipoch/medical-research-skills) into .agents/skills/buffer-calculator in your project. Codex loads it when a task matches its description.
Can I use Buffer Calculator in Cursor, Gemini CLI or GitHub Copilot?
Cursor, Gemini CLI, GitHub Copilot and OpenCode also load SKILL.md folders. With the skills CLI, run `npx skills add aipoch/medical-research-skills --skill buffer-calculator -a cursor` (or -a gemini-cli, github-copilot or opencode for the others). To copy it by hand, put the folder in .cursor/skills/buffer-calculator, .gemini/skills/buffer-calculator, .github/skills/buffer-calculator and .opencode/skills/buffer-calculator in your project.
What does Buffer Calculator need to run?
Going by SKILL.md and its folder, Buffer Calculator needs Python for the scripts in its folder and the command-line tools its instructions call (python). Our summary lists: Python 3.
Does Buffer Calculator access the network?
SKILL.md names 2 domains. As links in the text: cshprotocols.org and thermofisher.com. This is read from the text; nothing was executed.
Is Buffer Calculator safe to install?
Our automated static check of SKILL.md found no risky patterns, such as piping downloads into a shell, reading credential files or hidden Unicode. It is not a guarantee. The check reads SKILL.md only: the scripts in the folder are not scanned, so read them before running anything.
What licence does Buffer Calculator use?
Buffer Calculator is published under the MIT licence (declared in SKILL.md). It allows redistribution, so the full SKILL.md is shown on this page.
How many tokens does Buffer Calculator use?
About 1.8k tokens (SKILL.md is roughly 7.1k characters). Agents keep only the skill's name and description in context until a task matches; then they load SKILL.md in full.
What are the alternatives to Buffer Calculator?
Skills that share tags, products or a category with Buffer Calculator: Buffer (LeoYeAI/openclaw-master-skills, 2.2k stars), Measure Before You Fix (garrytan/gbrain, 31k stars), Eol Resistor Calculator (sickn33/agentic-awesome-skills, 47k stars) and Material Density Volume Calculation (InternScience/scp, 169 stars). The comparison table on this page puts their stars, adoption, token cost, safety result and licence side by side.
Who maintains Buffer Calculator?
aipoch (a GitHub organization) maintains it in aipoch/medical-research-skills, which has 1,974 GitHub stars. The repository holds 567 skills in this directory. The repository was last updated on September 17, 2026.
Source: aipoch/medical-research-skills on GitHub. Facts on this page come from the repository at the commit we read; the author's words are quoted as theirs.