Alphagenome Single Variant Analysis
google-deepmind/science-skills
Analyzes genetic variant effects on gene expression (RNA-seq), chromatin accessibility (DNASE), histone marks (ChIP), and transcription factors using the AlphaGenome API.
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments
Install Sgrna Design Guide
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a claude-codeProject install by default; add -g for ~/.claude/skills/.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guide --agent claude-codeProject scope by default; add --scope user for a personal install. Needs GitHub CLI 2.90.0 or later (public preview).
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .claude/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .claude/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.claude/skills/ instead of .claude/skills for a personal install. The folder must contain SKILL.md.
Claude Code skills documentation · loads skills from .claude/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .claude/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.Claude Code copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$skill-installer install https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guideType this inside Codex. $skill-installer <name> installs a curated skill from openai/skills. The installer writes to $CODEX_HOME/skills (default ~/.codex/skills). Restart Codex if the skill does not show up.
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a codexProject install goes to .agents/skills/; add -g for ~/.codex/skills/.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guide --agent codexProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .agents/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .agents/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.agents/skills/ instead of .agents/skills for a personal install.
Codex skills documentation · loads skills from .agents/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .agents/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.Codex copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a cursorProject install goes to .agents/skills/; add -g for ~/.cursor/skills/.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guide --agent cursorProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .cursor/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .cursor/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.cursor/skills/ instead of .cursor/skills for a personal install.
Cursor skills documentation · loads skills from .cursor/skills/, .agents/skills/, .claude/skills/, .codex/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .cursor/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.Cursor copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gemini skills install https://github.com/jaechang-hits/SciAgent-Skills.git --path skills/molecular-biology/sgrna-design-guide--scope user (default) or --scope workspace; --path is the subfolder of the repo that holds the skill; --consent skips the security confirmation prompt.
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a gemini-cliProject install goes to .agents/skills/; add -g for ~/.gemini/skills/.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guide --agent gemini-cliProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .gemini/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .gemini/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.gemini/skills/ instead of .gemini/skills for a personal install, then run /skills reload.
Gemini CLI skills documentation · loads skills from .gemini/skills/, .agents/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .gemini/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.Gemini CLI copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guideInstalls for Copilot at project scope by default; add --scope user for a personal install. Preview a skill first with gh skill preview. Needs GitHub CLI 2.90.0 or later (public preview).
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a github-copilotProject install goes to .agents/skills/; add -g for ~/.copilot/skills/.
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .github/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .github/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.copilot/skills/ instead of .github/skills for a personal install. Commit .github/skills so cloud agent and code review can use it.
GitHub Copilot skills documentation · loads skills from .github/skills/, .claude/skills/, .agents/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .github/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.GitHub Copilot copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
$ npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a opencodeOpenCode documents no install command of its own. Project install goes to .agents/skills/; add -g for ~/.config/opencode/skills/.
$ gh skill install jaechang-hits/SciAgent-Skills sgrna-design-guide --agent opencodeProject scope by default (.agents/skills/); add --scope user for a personal install.
$ git clone --depth 1 https://github.com/jaechang-hits/SciAgent-Skills.git skills-src && mkdir -p .opencode/skills && cp -r skills-src/skills/molecular-biology/sgrna-design-guide .opencode/skills/sgrna-design-guide && rm -rf skills-srcUse ~/.config/opencode/skills/ instead of .opencode/skills for a personal install.
OpenCode skills documentation · loads skills from .opencode/skills/, .claude/skills/, .agents/skills/
Install the "sgrna-design-guide" agent skill from https://github.com/jaechang-hits/SciAgent-Skills/tree/main/skills/molecular-biology/sgrna-design-guide into .opencode/skills/sgrna-design-guide/ in this project. Copy the whole folder (SKILL.md and every file beside it), keep the folder name "sgrna-design-guide", then confirm the skill loads.OpenCode copies the folder itself, the same result as the manual copy. Check what it changed before you commit it.
Facts
- Skill name
sgrna-design-guide- GitHub stars
- 374
- Used in
- 1 other repo
- Token cost
- ~6.2k tokens
- SKILL.md length
- 2,467 words
- Files
- 3
- Skills in repo
- 169
- Repo updated
- First seen
- Licence
- Custom licence
At a glance
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments
- Works in 7 steps: Understand File Naming Convention → Find Your Download URL → Download and Extract → …
- Tasks that involve Bioinformatics
- SKILL.md covers Metadata, Citations and Acknowledgments, Overview and Key Concepts, plus 8 more sections
- Calls wget; reaches portals.broadinstitute.org
What it does
Sgrna Design Guide is an agent skill from jaechang-hits/SciAgent-Skills. Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments
Its SKILL.md is about 6.2k tokens, which your agent loads only when the skill is triggered. The skill folder holds 3 other files.
It sits in Research & Science, covering Bioinformatics. The repository describes itself as: 197 bioinformatics & life science skills for Claude Code and AI agents — BixBench 92.0% accuracy. RNA-seq, single-cell, drug discovery, proteomics, and more. Powers OmicsHorizon.
When your agent uses it
- Tasks that involve Bioinformatics
Example prompts
- “/sgrna-design-guide”
Requirements
- Python 3
Workflow steps
7 steps, taken from the step headings in SKILL.md.
- Understand File Naming Convention
- Find Your Download URL
- Download and Extract
- Load Data and Filter for Your Gene
- Select Top sgRNAs
- Filter by Custom Criteria (Optional)
- Select Multiple sgRNAs for Validation
What it can do on your machine
Read from SKILL.md and the folder at commit 82c862c. It shows what the files ask for, not the result of running them.
Tool permissions
Pre-approves nothing: there is no allowed-tools line, so your agent's usual permission prompts apply.
From allowed-tools in the SKILL.md frontmatter.
Runs code
Shell commands in SKILL.md call:
wget
From the folder's file list and the shell code blocks in SKILL.md.
Network
Hosts in commands or code, which the agent is likely to contact:
portals.broadinstitute.org
Also links to:
doi.orgaddgene.org
From URLs in SKILL.md, links to its own repository left out.
Credentials
Names no API keys, tokens, secrets or passwords.
From names ending in _API_KEY, _TOKEN, _SECRET, _KEY or _PASSWORD in SKILL.md.
Context cost
Sgrna Design Guide loads about 6.2k tokens when it runs. Until then it costs about 41 tokens; SKILL.md has 2,467 words of instructions outside code blocks.
Estimates: characters ÷ 4, the usual rule of thumb; real counts depend on the model's tokenizer. Scripts and assets cost tokens only if the agent reads them.
Safety
Auto-check passed
The automated check found no risky patterns in SKILL.md.
Automated static check — not a guarantee. Review scripts before installing. It scans the text of SKILL.md for risky patterns (piping downloads into a shell, reading credential files, hidden Unicode, destructive commands); files beside SKILL.md are not scanned.
SKILL.md
Its licence (Custom licence) doesn't allow us to republish the file, so here is its outline and opening line. It has 2,467 words (~6,185 tokens).
“Short Description: Comprehensive guide for finding or designing sgRNAs using validated sequences, CRISPick datasets, or de novo design tools.”
- name
- sgrna-design-guide
- license
- open
Files
SKILL.md and 2 other files in skills/molecular-biology/sgrna-design-guide of jaechang-hits/SciAgent-Skills.
- SKILL.md
- resource/CRISPick_download_links.txt
- resource/addgene_grna_sequences.csv
Open the folder on GitHubat commit 82c862c
Used in 1 other repository
We found 1 copy of this SKILL.md (exact, near-identical or edited) in other folders, from 1 other GitHub owner. This page covers the copy in jaechang-hits/SciAgent-Skills, which our catalogue first saw on October 7, 2026.
Compare with similar skills
Sgrna Design Guide next to the 5 skills that share the most tags, products or categories with it. Stars are the repository's; “used in” counts other GitHub owners with a copy.
| Skill | Stars | Used in | Tokens | Auto-check | Licence | Repo updated |
|---|---|---|---|---|---|---|
| Sgrna Design Guide this skilljaechang-hits/SciAgent-Skills | 374 | 1 repos | ~6.2k | Automated safety check: Pass | Custom licence | |
| Alphagenome Single Variant Analysisgoogle-deepmind/science-skills | 3.2k | 2 repos | ~3k | Automated safety check: Notes | Apache-2.0 | |
| 13C Metabolic Flux AnalysisK-Dense-AI/scientific-agent-skills | 48k | 1 repos | ~3.2k | Automated safety check: Pass | MIT | |
| Clinvar Databasegoogle-deepmind/science-skills | 3.2k | 2 repos | ~3.9k | Automated safety check: Notes | Apache-2.0 | |
| Metabolic Study Planneraiming-lab/AutoResearchClaw | 15k | — | ~1.9k | Automated safety check: Pass | MIT | |
| Dbsnp Databasegoogle-deepmind/science-skills | 3.2k | 2 repos | ~3.4k | Automated safety check: Notes | Apache-2.0 |
Similar skills
13C Metabolic Flux Analysis
K-Dense-AI/scientific-agent-skills
Estimates reaction fluxes inside cells from steady-state carbon-13 labeling data with a bundled mfapy-based solver, and reports which fluxes the data pin down.
Clinvar Database
google-deepmind/science-skills
A skill your agent uses when needing clinical significance, pathogenicity classifications (e.g., Pathogenic, Benign, VUS), clinical evidence rationales, or finding "hard positive" benchmark controls…
Metabolic Study Planner
aiming-lab/AutoResearchClaw
Turns a broad metabolic modelling topic into a concrete, paper-shaped plan with organism, model, perturbations, metrics and figures before any FBA code is written.
Dbsnp Database
google-deepmind/science-skills
A skill your agent uses when you want to look up, map, and search for short genetic variants (SNPs, indels) in NCBI's dbSNP database.
MFA Pipeline Orchestrator
aiming-lab/AutoResearchClaw
Runs a metabolic flux analysis from model loading to phenotype prediction and figures by handing work to four sub-agents in sequence.
More from jaechang-hits/SciAgent-Skills
All 169 skills in this repoNeb Irc Activation Energy
jaechang-hits/SciAgent-Skills
NEB-IRC activation energy pipeline for reaction barriers using GFN2-xTB and pysisyphus.
Molecular Visualization 3dmol
jaechang-hits/SciAgent-Skills
3Dmol.js WebGL molecular visualization emitted as self-contained HTML.
Cobrapy Metabolic Modeling
jaechang-hits/SciAgent-Skills
Constraint-based (COBRA) analysis of genome-scale metabolic models: FBA, FVA, knockouts, flux sampling, production envelopes, gapfilling, media optimization.
Rdkit Chemdraw Cdxml
jaechang-hits/SciAgent-Skills
Read, write, and edit ChemDraw CDX/CDXML files with RDKit's rdkit.Chem.rdChemDraw plus direct XML editing, always paired with a rendered PNG.
Pubmed Database
jaechang-hits/SciAgent-Skills
Programmatic PubMed access via NCBI E-utilities REST API. An agent skill from jaechang-hits/SciAgent-Skills.
Sciagent Skill Creator
jaechang-hits/SciAgent-Skills
Scaffold a new SciAgent-Skills entry. An agent skill from jaechang-hits/SciAgent-Skills.
Related
Categories
Questions about Sgrna Design Guide
What does Sgrna Design Guide do?
Three-tiered sgRNA design guide using validated Addgene sequences, CRISPick pre-computed datasets, or de novo design rules for CRISPR experiments. Sgrna Design Guide is an agent skill from jaechang-hits/SciAgent-Skills.
When should I use Sgrna Design Guide?
Sgrna Design Guide fits situations like: tasks that involve Bioinformatics.
How do I install Sgrna Design Guide in Claude Code?
Run `npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a claude-code`. Or copy the skill folder (skills/molecular-biology/sgrna-design-guide in jaechang-hits/SciAgent-Skills) into .claude/skills/sgrna-design-guide in your project. Claude Code loads it when a task matches its description.
How do I install Sgrna Design Guide in Codex?
Run `npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a codex`. Or copy the skill folder (skills/molecular-biology/sgrna-design-guide in jaechang-hits/SciAgent-Skills) into .agents/skills/sgrna-design-guide in your project. Codex loads it when a task matches its description.
Can I use Sgrna Design Guide in Cursor, Gemini CLI or GitHub Copilot?
Cursor, Gemini CLI, GitHub Copilot and OpenCode also load SKILL.md folders. With the skills CLI, run `npx skills add jaechang-hits/SciAgent-Skills --skill sgrna-design-guide -a cursor` (or -a gemini-cli, github-copilot or opencode for the others). To copy it by hand, put the folder in .cursor/skills/sgrna-design-guide, .gemini/skills/sgrna-design-guide, .github/skills/sgrna-design-guide and .opencode/skills/sgrna-design-guide in your project.
What does Sgrna Design Guide need to run?
Going by SKILL.md and its folder, Sgrna Design Guide needs the command-line tools its instructions call (wget). Our summary lists: Python 3.
Does Sgrna Design Guide access the network?
SKILL.md names 3 domains. In commands or code: portals.broadinstitute.org; the agent is likely to contact it when it follows the instructions. As links in the text: doi.org and addgene.org. This is read from the text; nothing was executed.
Is Sgrna Design Guide safe to install?
Our automated static check of SKILL.md found no risky patterns, such as piping downloads into a shell, reading credential files or hidden Unicode. It is not a guarantee. Review the folder before installing.
What licence does Sgrna Design Guide use?
Sgrna Design Guide has a licence file (the repository's licence) that doesn't match a standard licence. Read it on GitHub before reusing the skill.
How many tokens does Sgrna Design Guide use?
About 6.2k tokens (SKILL.md is roughly 25k characters). Agents keep only the skill's name and description in context until a task matches; then they load SKILL.md in full.
What are the alternatives to Sgrna Design Guide?
Skills that share tags, products or a category with Sgrna Design Guide: Alphagenome Single Variant Analysis (google-deepmind/science-skills, 3.2k stars), 13C Metabolic Flux Analysis (K-Dense-AI/scientific-agent-skills, 48k stars), Clinvar Database (google-deepmind/science-skills, 3.2k stars) and Metabolic Study Planner (aiming-lab/AutoResearchClaw, 15k stars). The comparison table on this page puts their stars, adoption, token cost, safety result and licence side by side.
Who maintains Sgrna Design Guide?
jaechang-hits (a GitHub user) maintains it in jaechang-hits/SciAgent-Skills, which has 374 GitHub stars. The repository holds 169 skills in this directory. The repository was last updated on September 29, 2026.
Source: jaechang-hits/SciAgent-Skills on GitHub. Facts on this page come from the repository at the commit we read; the author's words are quoted as theirs.