Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller.

No licenceAuto-check passed

Install Bio Basecalling

skills CLI
$ npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-basecalling -a claude-code

Project install by default; add -g for ~/.claude/skills/.

GitHub CLI
$ gh skill install FreedomIntelligence/OpenClaw-Medical-Skills bio-basecalling --agent claude-code

Project scope by default; add --scope user for a personal install. Needs GitHub CLI 2.90.0 or later (public preview).

Manual copy
$ git clone --depth 1 https://github.com/FreedomIntelligence/OpenClaw-Medical-Skills.git skills-src && mkdir -p .claude/skills && cp -r skills-src/skills/bio-basecalling .claude/skills/bio-basecalling && rm -rf skills-src

Use ~/.claude/skills/ instead of .claude/skills for a personal install. The folder must contain SKILL.md.

Claude Code skills documentation · loads skills from .claude/skills/

Facts

Skill name
bio-basecalling
GitHub stars
3.1k
Used in
1 other repo
Token cost
~2k tokens
SKILL.md length
384 words
Files
4
Skills in repo
173
Repo updated
First seen
Licence
None found

At a glance

Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller.

  • Processing raw Nanopore data before alignment
  • SKILL.md covers Version Compatibility, Dorado (Recommended), Guppy (Deprecated - Legacy Only) and POD5 File Handling
  • Runs Shell scripts from its folder

What it does

Bio Basecalling is an agent skill from FreedomIntelligence/OpenClaw-Medical-Skills. Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Covers model selection, GPU acceleration, modified base detection, and quality filtering. Use when processing raw Nanopore data before alignment. Guppy is deprecated; use Dorado for all new analyses.

Its SKILL.md is about 2k tokens, which your agent loads only when the skill is triggered. The skill folder holds 4 other files (for example `examples/basecall_pipeline.sh`, `examples/dorado_basecall.sh` and `usage-guide.md`).

The repository describes itself as: The largest open-source medical AI skills library for OpenClaw🦞.

When your agent uses it

  • Processing raw Nanopore data before alignment

Example prompts

  • “/bio-basecalling”

Requirements

  • A Bash shell

What it can do on your machine

Read from SKILL.md and the folder at commit b1f9b6e. It shows what the files ask for, not the result of running them.

  • Tool permissions

    Pre-approves nothing: there is no allowed-tools line, so your agent's usual permission prompts apply.

    From allowed-tools in the SKILL.md frontmatter.

  • Runs code

    Ships script files (Shell), which the agent can run.

    From the folder's file list and the shell code blocks in SKILL.md.

  • Network

    No URLs in SKILL.md.

    From URLs in SKILL.md, links to its own repository left out.

  • Credentials

    Names no API keys, tokens, secrets or passwords.

    From names ending in _API_KEY, _TOKEN, _SECRET, _KEY or _PASSWORD in SKILL.md.

Context cost

Bio Basecalling loads about 2k tokens when it runs. Until then it costs about 78 tokens; SKILL.md has 384 words of instructions outside code blocks.

Always · name and description, kept in context so the agent knows when to use it
~78
When it runs · the whole SKILL.md, loaded when a task matches
~2k

Estimates: characters ÷ 4, the usual rule of thumb; real counts depend on the model's tokenizer. Scripts and assets cost tokens only if the agent reads them.

Safety

Auto-check passed

The automated check found no risky patterns in SKILL.md.

Automated static check — not a guarantee. Review scripts before installing. It scans the text of SKILL.md for risky patterns (piping downloads into a shell, reading credential files, hidden Unicode, destructive commands); files beside SKILL.md are not scanned.

SKILL.md

Without a licence we can't republish the file, so here is its outline and opening line. It has 384 words (~2,039 tokens).

“Reference examples tested with: samtools 1.19+”

— opening of SKILL.md by FreedomIntelligence
name
bio-basecalling
tool_type
cli
primary_tool
dorado

Read the full SKILL.md on GitHub

Files

SKILL.md and 3 other files in skills/bio-basecalling of FreedomIntelligence/OpenClaw-Medical-Skills.

  • SKILL.md
  • examples/basecall_pipeline.sh
  • examples/dorado_basecall.sh
  • usage-guide.md

Open the folder on GitHubat commit b1f9b6e

Used in 1 other repository

We found 1 copy of this SKILL.md (exact, near-identical or edited) in other folders, from 1 other GitHub owner. This page covers the copy in FreedomIntelligence/OpenClaw-Medical-Skills, which our catalogue first saw on October 7, 2026.

Compare with similar skills

Bio Basecalling next to the 5 skills that share the most tags, products or categories with it. Stars are the repository's; “used in” counts other GitHub owners with a copy.

Bio Basecalling compared with similar skills
SkillStarsUsed inTokensAuto-checkLicenceRepo updated
Bio Basecalling this skillFreedomIntelligence/OpenClaw-Medical-Skills3.1k1 repos~2kAutomated safety check: PassNone
Convertremotion-dev/remotion62k—~247Automated safety check: PassCustom licence
Bio Long Read Sequencing BasecallingGPTomics/bioSkills1.2k1 repos~3.5kAutomated safety check: PassMIT
Trader Signalruvnet/ruflo74k—~605Automated safety check: NotesMIT
Waypoint BioK-Dense-AI/scientific-agent-skills48k1 repos~4.2kAutomated safety check: PassMIT
Agent Signal Pipelinelobehub/lobehub83k—~1.7kAutomated safety check: PassCustom licence

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Questions about Bio Basecalling

What does Bio Basecalling do?

Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller. Bio Basecalling is an agent skill from FreedomIntelligence/OpenClaw-Medical-Skills. Convert raw Nanopore signal data (FAST5/POD5) to nucleotide sequences using Dorado basecaller.

When should I use Bio Basecalling?

Bio Basecalling fits situations like: processing raw Nanopore data before alignment.

How do I install Bio Basecalling in Claude Code?

Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-basecalling -a claude-code`. Or copy the skill folder (skills/bio-basecalling in FreedomIntelligence/OpenClaw-Medical-Skills) into .claude/skills/bio-basecalling in your project. Claude Code loads it when a task matches its description.

How do I install Bio Basecalling in Codex?

Run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-basecalling -a codex`. Or copy the skill folder (skills/bio-basecalling in FreedomIntelligence/OpenClaw-Medical-Skills) into .agents/skills/bio-basecalling in your project. Codex loads it when a task matches its description.

Can I use Bio Basecalling in Cursor, Gemini CLI or GitHub Copilot?

Cursor, Gemini CLI, GitHub Copilot and OpenCode also load SKILL.md folders. With the skills CLI, run `npx skills add FreedomIntelligence/OpenClaw-Medical-Skills --skill bio-basecalling -a cursor` (or -a gemini-cli, github-copilot or opencode for the others). To copy it by hand, put the folder in .cursor/skills/bio-basecalling, .gemini/skills/bio-basecalling, .github/skills/bio-basecalling and .opencode/skills/bio-basecalling in your project.

What does Bio Basecalling need to run?

Going by SKILL.md and its folder, Bio Basecalling needs a shell for the scripts in its folder. Our summary lists: A Bash shell.

Does Bio Basecalling access the network?

SKILL.md contains no URLs. Any network use would come from the scripts or tools the agent runs. This is read from the text; nothing was executed.

Is Bio Basecalling safe to install?

Our automated static check of SKILL.md found no risky patterns, such as piping downloads into a shell, reading credential files or hidden Unicode. It is not a guarantee. Review the folder before installing.

What licence does Bio Basecalling use?

No licence was found for Bio Basecalling or its repository. Without one, default copyright applies: ask the author before reusing or redistributing it.

How many tokens does Bio Basecalling use?

About 2k tokens (SKILL.md is roughly 8.2k characters). Agents keep only the skill's name and description in context until a task matches; then they load SKILL.md in full.

What are the alternatives to Bio Basecalling?

Skills that share tags, products or a category with Bio Basecalling: Convert (remotion-dev/remotion, 62k stars), Bio Long Read Sequencing Basecalling (GPTomics/bioSkills, 1.2k stars), Trader Signal (ruvnet/ruflo, 74k stars) and Waypoint Bio (K-Dense-AI/scientific-agent-skills, 48k stars). The comparison table on this page puts their stars, adoption, token cost, safety result and licence side by side.

Who maintains Bio Basecalling?

FreedomIntelligence (a GitHub organization) maintains it in FreedomIntelligence/OpenClaw-Medical-Skills, which has 3,051 GitHub stars. The repository holds 173 skills in this directory. The repository was last updated on July 21, 2026.

Source: FreedomIntelligence/OpenClaw-Medical-Skills on GitHub. Facts on this page come from the repository at the commit we read; the author's words are quoted as theirs.