Research & Science · By TianGzlab

Load when the user needs Deterministic fixed-period 24-hour single-component cosinor OLS rhythm analysis for a bulk RNA time-course CSV.

TianGzlab/OmicsClaw

Load when correcting batch effects in bulk expression using R sva ComBat or the legacy Python parametric approximation.

TianGzlab/OmicsClaw

Load when discovering bulk gene co-expression modules and hub genes with R WGCNA.

TianGzlab/OmicsClaw

Load when comparing gene expression between two conditions in bulk RNA-seq count data.

TianGzlab/OmicsClaw

Load when estimating cell-type proportions in bulk RNA-seq samples from a single-cell or signature-matrix reference.

TianGzlab/OmicsClaw

Load when running pathway / GO term enrichment on a bulk RNA-seq DE result list.

TianGzlab/OmicsClaw

Load when converting Ensembl, Entrez or symbol IDs in a bulk RNA count matrix using an explicit mapping or a small human demo reference.

TianGzlab/OmicsClaw

Load when querying STRING for the protein-protein interaction neighborhood of a bulk RNA-seq DEG list and finding hub genes.

TianGzlab/OmicsClaw

Load when checking a bulk RNA-seq count matrix for library-size outliers, gene detection rates, and sample-sample correlation before DE.

TianGzlab/OmicsClaw

Load when summarising STAR / HISAT2 / Salmon alignment-rate logs in bulk RNA-seq.

TianGzlab/OmicsClaw

Load when checking raw FASTQ quality (Phred / GC / adapter / Q20-Q30) before alignment in bulk RNA-seq.

TianGzlab/OmicsClaw

Load when summarising rMATS / SUPPA2 alternative-splicing output and identifying significant differential splicing events.

TianGzlab/OmicsClaw

Load when placing bulk RNA-seq samples on a single-cell reference's pseudotime axis (NNLS deconvolution + nearest-neighbour mapping).

TianGzlab/OmicsClaw

Load when computing alignment QC metrics (mapping rate, MAPQ distribution, insert size, duplicate rate, proper-pair rate) from a text SAM file produced by any short-/long-read aligner (BWA / Bowtie2…

TianGzlab/OmicsClaw

Load when computing genome-assembly QC metrics — N50/N90, L50/L90, total length, contig count, GC content, longest-contig — from a FASTA produced by any assembler (SPAdes / Megahit / Flye / Canu).

TianGzlab/OmicsClaw

Load when calling CNV segments via CBS-style segmentation on a bin-level log2-ratio CSV from exome / WGS coverage — emits per-segment 5-class CN state (amplification / gain / neutral / loss /…

TianGzlab/OmicsClaw

Load when summarising a peak file (BED / narrowPeak) from ATAC-seq / ChIP-seq / CUT&Tag — peak count, width distribution, per-chromosome counts, score statistics.

TianGzlab/OmicsClaw

Load when summarising a phased VCF (output of WhatsHap / SHAPEIT5 / Eagle2) — phased fraction of het variants, phase-block N50, PS-field parsing, pipe-delimited genotype detection.

TianGzlab/OmicsClaw

Load when running pre-alignment FASTQ quality control — Phred quality scores, Q20/Q30 rates, GC / N content, read-length distribution, adapter-contamination detection.

TianGzlab/OmicsClaw

Load when summarising structural variants from an SV VCF (DEL / DUP / INV / TRA) — INFO/SVTYPE-based classification, size classification, per-type counts.

TianGzlab/OmicsClaw

Load when summarising functional impact of an annotated variant CSV — per-IMPACT counts (HIGH / MODERATE / LOW / MODIFIER), top consequences, gene-affected count.

TianGzlab/OmicsClaw

Load when summarising small variants (SNVs / indels) from a VCF or computing demo-pattern variant statistics (Ti/Tv ratio, per-chromosome distribution, SNP / indel split).

TianGzlab/OmicsClaw

Load when summarising / filtering a VCF — variant classification (SNP / MNP / INS / DEL / COMPLEX), Ti/Tv ratio, QUAL / DP threshold filtering, INFO-field parsing.

TianGzlab/OmicsClaw

Load when extracting GEO accessions, dataset metadata, and downloadable references from a scientific paper (PDF / URL / DOI / PubMed ID / raw text) for downstream omics analysis.

TianGzlab/OmicsClaw

Load when ingesting a MaxQuant proteinGroups.txt, FragPipe combinedprotein.tsv, DIA-NN report, or generic CSV / TSV protein-quantification table — normalises columns to a standard schema, emits…

TianGzlab/OmicsClaw

Load when computing two-group differential protein abundance (group2 vs group1, log2FC + p-value + BH-adjusted FDR) via Welch t-test, equal-variance t-test, or Mann-Whitney on a wide protein ×…

TianGzlab/OmicsClaw

Load for Fisher over-representation analysis of protein identifiers against caller-supplied pathways.

TianGzlab/OmicsClaw

Load when summarising peptide identifications (PSM count, unique peptide count, distinct protein count, score / charge distributions) from a peptide-level CSV produced by MaxQuant / FragPipe / DIA-NN.

TianGzlab/OmicsClaw

Load when computing protein-table QC — proteins × samples count, missing-value rate, intensity CV (median + mean) — from a MaxQuant / FragPipe / DIA-NN protein-quantification CSV.

TianGzlab/OmicsClaw

Load when summarising PTM sites (phosphorylation, acetylation, ubiquitination, etc.) from a per-site CSV — site-class assignment (Olsen et al.

TianGzlab/OmicsClaw

Load when computing per-protein abundance from a peptide / PSM table via LFQ (intensity summation), iBAQ (intensity / tryptic peptide count), or spectral counting (PSMs per protein).

TianGzlab/OmicsClaw

Load when summarising cross-linking MS (XL-MS) results — intra/inter-protein link split, optional FDR filtering, distance-constraint validation against a per-crosslinker (DSS / BS3 / EDC / DSSO /…

TianGzlab/OmicsClaw

Load when removing ambient RNA contamination from droplet-based scRNA-seq using a simple subtraction path, CellBender, or SoupX.

TianGzlab/OmicsClaw

Load when integrating multi-sample scRNA-seq with Harmony, scVI, scANVI, BBKNN, Scanorama, SIMBA, or supported R-backed methods to remove batch effects.

TianGzlab/OmicsClaw

Load when assigning cell-type labels to a clustered scRNA AnnData via marker dictionaries, CellTypist, PopV, KNNPredict, SingleR, scmap, SCSA, or a manual cluster-to-label map.

TianGzlab/OmicsClaw

Load when computing cell-cell ligand-receptor communication on an annotated scRNA AnnData via builtin scorer, LIANA, CellPhoneDB, CellChat (R), or NicheNet (R).

TianGzlab/OmicsClaw

Load when building the neighbour graph, embedding (UMAP/t-SNE/diffmap/PHATE), and clustering (Leiden/Louvain) on a normalised single-cell AnnData.

TianGzlab/OmicsClaw

Load when turning scRNA FASTQ (or existing CellRanger/STARsolo/SimpleAF/kb-python output) into a downstream-ready AnnData.

TianGzlab/OmicsClaw

Load when computing per-cell differentiation potency / stemness scores from gene-expression complexity on a scRNA AnnData via the CytoTRACE-simple method.

TianGzlab/OmicsClaw

Load when finding marker genes per cluster or comparing condition expression in single-cell RNA-seq.

TianGzlab/OmicsClaw

Load when testing whether cell-type / cluster proportions or neighbourhood densities differ between conditions in a multi-sample scRNA AnnData via Milo, scCODA, simple proportion screen, or R…

TianGzlab/OmicsClaw

Load when annotating putative doublets in single-cell RNA-seq using Scrublet, DoubletDetection, DoubletFinder, scDblFinder, or scds.

TianGzlab/OmicsClaw

Load when scoring drug sensitivity per cluster on an annotated scRNA AnnData via simple-correlation against drug-target signatures or via CaDRReS-Sc pretrained models (GDSC / PRISM).

TianGzlab/OmicsClaw

Load when running bulk-style pathway enrichment (ORA / GSEA / GSEA-R / GSVA-R) on a per-group ranked DE / marker list against a gene-set library.

TianGzlab/OmicsClaw

Load when checking raw single-cell FASTQ read quality (Phred / GC / adapter / length) before counting.

TianGzlab/OmicsClaw

Load when removing low-quality cells and lowly-detected genes from a single-cell AnnData using QC-derived thresholds or tissue presets.

TianGzlab/OmicsClaw

Load when extracting gene programs (NMF / cNMF factorisation) and per-cell program usage scores from a non-negative scRNA AnnData.

TianGzlab/OmicsClaw

Load when inferring TF → target gene regulatory networks on a normalised scRNA AnnData via pySCENIC (GRNBoost2 + cisTarget + AUCell) or correlation-based GRN fallback (when arboreto is unavailable…

TianGzlab/OmicsClaw